Can 16-bit software run in Windows 2000 platform?
The answer is yes and no.
Many 16-bit software programs run just fine in W2K, as they did in NT 3.5 and 4.0. I still use Vernon Buerg's LIST program. But as in earlier versions of NT programs that try to make direct calls to hardware will fail, because NT requires that these be routed through a virtual layer. Many of the earlier (and later) games fall into this category. There are other reasons that some 16-bit programs will not run on NT or W2K, including those that require specific drivers.
So, the best way to discover if a particular program will run is to try it.